Table. 2.

Secretion of active renin from As4.1 cells during 100% confluence day 1 to 4

Post-confluent day (ng ANG I/well/day)
Day 1 Day 2 Day 3 Day 4
DMEM 2.68 ± 1.03 10.00 ± 3.53 6.94 ± 2.82 3.10 ± 0.47
DMEM + 10% FBS 5.33 ± 1.74* 9.00 ± 5.12 13.40 ± 1.65 8.29 ± 4.04*

Values are means ± SD from 6 wells. When cells grown to 100% confluent, incubation media were switched with fresh media of DMEM or DMEM + 10% FBS every day, and incubated for 1 h. Secreted renin activity was determined as described in Table 1. ANG I, angiotensin I; DMEM, Dulbecco's modified eagle medium; FBS, fetal bovine serum. *p < 0.005 DMEM vs. DMEM + 10% FBS.

Korean J Physiol Pharmacol 2022;26:479-499 https://doi.org/10.4196/kjpp.2022.26.6.479
© Korean J Physiol Pharmacol