Table. 1.

Secretion of active renin and inactive renin (prorenin) from preconfluent As4.1 cells in the absence and presence of ML-7

ng ANG I/well/h Inactive/total (%)
Total Active Inactive
DMEM + 10% FBS 1,486 ± 209 36.8 ± 4.5 1,449 ± 209 97.5 ± 0.4
DMEM + 10% FBS + ML-7 1,502 ± 209 26.9 ± 2.9 1,475 ± 220 98.2 ± 0.4

Values represent means ± SD from 6 wells. Preconfluent (70%–80% confluence) As4.1cells were incubated in DMEM + 10% FBS in the absence and presence of ML-7 (6 × 10−5 M) for 1 h each at 37°C in a humidified atmosphere of 5% CO2. Collected media were centrifuged at 1,000 ×g for 10 min at 4°C to remove detached cells. Renin activity was determined with (total) and without (active) trypsin (1 mg/ml) after 30 min on ice. Inactive renin was calculated by subtracting active renin from the total. ANG I, angiotensin I; DMEM, Dulbecco's modified eagle medium; FBS, fetal bovine serum. No significant difference before vs. after ML-7 by paired Student’s t-test.

Korean J Physiol Pharmacol 2022;26:479-499 https://doi.org/10.4196/kjpp.2022.26.6.479
© Korean J Physiol Pharmacol