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Fig. 1. Growth curve and cadherin expression before (lane 1) and after confluence (lane 2). (A) Growth curve. Cells were plated (3 × 103 cells/well) onto 24-well plates (day 0), cultured for 10 days and counted on a hemocytometer every 2–3 days (n = 3). (B) Phase contrast microscopy of ~70% and 100% confluent cells (×200). Scale bar: 60 μm. (C) Expression of cadherins. Protein (40 μg) obtained from cell lysates of cells grown to ~70% confluence (lane 1) or from cells on day 2 postconfluence (lane 2) were resolved by 7% acrylamide SDS-PAGE, followed by Western blotting with antibodies against epithelial cadherin (E-cad), placental cadherin (P-Cad), or neural cadherin (N-Cad). The numbers on the left indicate the molecular masses of standard proteins in kilo Daltons (kDa). The density of the bands was compared by unpaired Student’s tests (n = 3 for each group). β-actin was used as the protein loading control for each gel. NS, no significant difference. (D) N-cad expression at ~70% confluence (upper panel) and 100% confluence (lower panel). Cell nuclei were stained with propidium iodide (DAPI). Scale bar: 10 μm.
Korean J Physiol Pharmacol 2022;26:479-499
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