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Fig. 2. Valdecoxib (Val) inhibited FaDu cell migration and invasion. FaDu cells were seeded onto coverslips in 24-well plates for 24 h. After cells reached 80% confluence, a scratch (5 mm) was made with a 10 μl pipette tip, and then cells were treated with Val (0, 50, and 75 μM) for 48 h. Images of the wound were taken using a microscope (A: ×100, magnification) and the degree of wound closure was measured (B). The transwell assay was performed to identify the migrating and invading cells using 8 μm cell hanging inserts. Cells were stained with crystal violet and images were captured using a microscope (C: ×100 magnification). To quantify the percentage of migrated and invaded cells, they were dissolved in 10% cetylpyridinium chloride and the optical density was recorded using a microplate photometer (D). Vertical bars indicate means and standard errors (n = 3).
Korean J Physiol Pharmacol 2022;26:439-446 https://doi.org/10.4196/kjpp.2022.26.6.439
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