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Fig. 4. Effect of discoid domain receptor 1 (DDR1) knockdown on HK-2 cell pyroptosis. (A) Bands corresponding to the expression of DDR1 in the background of transfection with siDDR1. (B) Quantification of the band observed in (B). (C) Propidium iodide (PI) staining was used to analyze HK-2 cell death (scale bar: 200 μm). (D) Percentage HK-2 cell pyroptosis. (E) Bands corresponding to the expression of pyroptosis-related proteins (caspase-1 p45, cleaved caspase-1, interleukin [IL]-1β, IL-18, gasdermin D [GSDMD], and GSDMD-N). (F) Quantification of the bands observed in (E). (G) The concentration of IL-1β in HK-2 cell supernatant. (H) The concentration of IL-18 in HK-2 cell supernatant. Data are presented as mean ± SEM from 3 replicated cell experiments. Compared to high glucose (HG), *p < 0.05, **p < 0.01.
Korean J Physiol Pharmacol 2022;26:427-438 https://doi.org/10.4196/kjpp.2022.26.6.427
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