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Fig. 2. In vivo and in vitro expression of pyroptosis-related proteins. (A) Bands corresponding to the expression of pyroptosis-related proteins (caspase-1 p45, cleaved caspase-1, interleukin [IL]-1β, IL-18, gasdermin D [GSDMD], and GSDMD-N) in the kidneys of diabetic nephropathy (DN) rats. (B) Quantification of the bands observed in (A). (C) Concentration of IL-1β in rat plasma. (D) Concentration of IL-18 in rat plasma. (E) Bands corresponding to the expression of pyroptosis-related proteins (caspase-1 p45, cleaved caspase-1, IL-1β, IL-18, GSDMD, and GSDMD-N) in high glucose (HG)-treated HK-2 cells. (F) Quantification of the bands observed in (E). (G) Concentration of IL-1β in HK-2 cell supernatant. (H) Concentration of IL-18 in HK-2 cell supernatant. Data are presented as mean ± SEM from 6 rats and 3 replicated cell experiments. Compared to control, *p < 0.05, **p < 0.01.
Korean J Physiol Pharmacol 2022;26:427-438 https://doi.org/10.4196/kjpp.2022.26.6.427
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