Table. 2.

Time course incorporation of L-[14C(U)]-Leucine into protein in serially subcultured human fibroblasts (cpm/μg protein)

Hours post-subculture Subculture passage
P5 P25 P35
12 18.2 ± 0.35 7.1 ± 0.24 8.3 ± 0.30
24 21.2 ± 0.53 6.8 ± 0.25 8.6 ± 0.29
48 20.6 ± 0.51 8.3 ± 0.26 11.4 ± 0.38
72 21.3 ± 0.58 13.1 ± 0.40 15.2 ± 0.41
96 20.9 ± 0.50 19.7 ± 0.48 17.3 ± 0.43
120 21.4 ± 0.55 21.3 ± 0.54 20.5 ± 0.51
144 20.5 ± 0.57 20.9 ± 0.56 21.2 ± 0.58

Values are means ± SD of duplicate determinations in six subcultures.

Korean J Physiol Pharmacol 2022;26:263-275 https://doi.org/10.4196/kjpp.2022.26.4.263
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