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Fig. 8. MiR-21/PARP-1 regulates the EMT of 16HBE cells via activation of the PI3K/AKT signaling pathway. (A, B) Western blot analysis evaluated the protein expressions of PI3K/AKT-signaling-pathway-related molecular proteins in 16HBE cells transfected with NC, miR-21 mimics, or PARP-1 siRNA after 48 h. The experiments were replicated three times and presented as mean ± SD. *p < 0.05 vs. NC + D group, **p < 0.001 vs. NC + D group, p < 0.05 vs. miR21 + D or siPARP-1 + D group. (C–F) Effects of LY294002 on the EMT-related proteins in 16HBE cells transfected with NC, miR-21 mimics, or PARP-1 siRNA, as detected by Western blot and qRT-PCR after 48 h. The experiments were replicated three times and presented as mean ± SD. miR-21, microRNA-21; PARP-1, poly (ADP-ribose) polymerase-1; EMT, epithelial-mesenchymal transition; 16HBE, Human bronchial epithelial cell; NC, normal control; siRNA, small interfering RNA; siPARP-1, PARP-1 siRNA; D, dimethylsulfoxide; ns, not significant; LY, LY294002. *p < 0.05 vs. NC + D group, **p < 0.01 vs. NC + D group, ***p < 0.001 vs. NC + D group, ****p < 0.0001 vs. NC + D group, p < 0.05 vs. miR21 + D or siPARP-1 + D group, ††p < 0.01 vs. miR21 + D or siPARP-1 + D group, †††p < 0.001 vs. miR21 + D or siPARP-1 + D group.
Korean J Physiol Pharmacol 2022;26:239-253 https://doi.org/10.4196/kjpp.2022.26.4.239
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