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Fig. 5. Overexpression of PARP-1 alleviates the EMT induced by miR-21 mimics in 16HBE cells. (A, C, E, G) Western blot analysis evaluated the protein expression of Snail, ZEB1, N-cadherin, Vimentin, and E-cadherin in 16HBE cells transfected with NC, miR-21 mimics, miR-21 inhibitor, miR-21 mimics + PARP-1 plasmid or miR-21 inhibitor + PARP-1 siRNA after 48 h. The experiments were replicated three times and presented as mean ± SD. *p < 0.05 vs. NC group, **p < 0.01 vs. NC group, p < 0.05 vs. miR21 or miR21 inhibitor group, ††p < 0.01, vs. miR21 or miR21 inhibitor group. (B, D, F, H) The mRNA expression of Snail, ZEB1, E-cadherin, Vimentin, and E-cadherin in 16HBE cells transfected with NC, miR-21 mimics, miR-21 inhibitor, miR-21 mimics + PARP-1 plasmid, or miR-21 inhibitor + PARP-1 siRNA after 48 h were detected by qRT-PCR. The experiments were replicated three times and presented as mean ± SD. PARP-1, poly (ADP-ribose) polymerase-1; EMT, epithelial-mesenchymal transition; miR-21, microRNA-21; 16HBE, Human bronchial epithelial cell; NC, normal control; siRNA, small interfering RNA; siPARP-1, PARP-1 siRNA. *p < 0.05 vs. NC group, **p < 0.01 vs. NC group, ***p < 0.001 vs. NC group, ****p < 0.0001 vs. NC group, p < 0.05 vs. miR21 inhibitor group, ††p < 0.01 vs. miR21 group, †††p < 0.001 vs. miR21 group.
Korean J Physiol Pharmacol 2022;26:239-253 https://doi.org/10.4196/kjpp.2022.26.4.239
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