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Fig. 4. Effects of PARP-1 on expression of EMT-related proteins and EMT-regulating transcription factors. (A, C) Western blot analysis evaluated the expression of Snail, ZEB1, N-cadherin, Vimentin and E-cadherin in 16HBE cells transfected with NC, PARP-1 plasmid, or PARP-1 siRNA after 48 h. The experiments were replicated three times and presented as mean ± SD. *p < 0.05 vs. NC group, **p < 0.01 vs. NC group. (B, D) The mRNA expressions of Snail, ZEB1, N-cadherin, Vimentin, and E-cadherin in 16HBE cells transfected with NC, PARP-1 plasmid, or PARP-1 siRNA after 48 h, as detected by qRT-PCR. The experiments were replicated three times and presented as mean ± SD. PARP-1, poly (ADP-ribose) polymerase-1; EMT, epithelial-mesenchymal transition; 16HBE, Human bronchial epithelial cell; NC, normal control; siRNA, small interfering RNA; siPARP-1, PARP-1 siRNA. *p < 0.05 vs. NC group, **p < 0.01 vs. NC group, ***p < 0.001 vs. NC group.
Korean J Physiol Pharmacol 2022;26:239-253 https://doi.org/10.4196/kjpp.2022.26.4.239
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