Fig. 4. ROCK is involved in LPC-induced fibrous (F)-actin polymerization in human neutrophils.
(A, B) Neutrophils were pretreated with Y27632 (10 μM) or vehicle for 30 min, and further stimulated with LPC (3 μM) for 15 min, and then were stained with FITC-conjugated phalloidin for F-actin polymerization. Neutrophils were analyzed with FACS (A) and immunofluorescence microscopy (bar, 2 μm) with a × 100 (scan zoom 3) objectives (B). An average ± SEM of more than three experiments is shown. ROCK, Rho kinase; LPC, lysophosphatidylcholine. **p < 0.01 and ***p < 0.001.
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