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Fig. 3. Rho is involved in LPC-induced CD63 surface expression in human neurophils. (A–C) Neutrophils were pretreated with NSC23766 (50 μM), Y27632 (10 μM), Tat-C3 exoenzyme (2.5–5 μg/ml), or vehicle for 30 min, and further stimulated with LPC (3 μM) for 15 min. (D) Rho activation in response to LPC in human neutrophils determined by pull-down assay. An average ± SEM of more than three experiments is shown. LPC, lysophosphatidylcholine; MFI, mean fluorescence intensity. *p < 0.05 and **p < 0.01.
Korean J Physiol Pharmacol 2022;26:175-182
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