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Fig. 2. LPC-induced CD63 surface expression via fibrous (F)-actin polymerization in human neutrophils. (A) Representative FACS histograms of CD63 surface expression in response to cytochalasin B (CB), fMLP, CB + fMLP, LPC, and CB + LPC. Isolated neutrophils stabilized 30 min were treated with CB (2.5 μg/ml, 5 min), fMLP (1 μM, 15 min), or LPC (3 μM, 15 min). When combined, neutrophils were pretreated with CB and then treated with either fMLP or LPC. (B) Bar graph shows mean fluorescence intensity (MFI) of more than three independent experiments. (C) FITC-conjugated phalloidin, a marker of F-actin, was measured after exposure of neutrophils to LPC for 15 min. An average ± SEM of more than three experiments is shown. LPC, lysophosphatidylcholine; fMLP, N-formyl-L-methionyl-L-leucyl-L-phenylalanine. *p < 0.05, **p < 0.01, and ***p < 0.001.
Korean J Physiol Pharmacol 2022;26:175-182
© Korean J Physiol Pharmacol