Fig. 1. Translocation of azurophil granule in response to LPC in human neutrophils.
(A, B) Time- (at 3 μM) and concentration- (at 15 min) dependency of LPC-induced CD63 surface expression. After fixation, cells were stained with FITC-conjugated antibody against CD63, an azurophil granule marker for 1 h on ice, and were analyzed with flow cytometry. An average ± SEM of more than three experiments is shown. LPC, lysophosphatidylcholine; MFI, mean fluorescence intensity. *p < 0.05 and **p < 0.01.
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