Fig. 5. Oxymatrine affected Nrf2, HO-1, and Keap1 levels in INS-1 cells.
(A) Representative Western blotting bands of Nrf2, HO-1, and Keap1 proteins in the nucleus or cytoplasm were showed. (B) Analysis of the amount of Nrf2 proteins in the nucleus. (C) Analysis of the amount of Nrf2 proteins in the cytoplasm. (D) Analysis of the amount of Keap1 proteins. (E) Analysis of the amount of HO-1 proteins. Control: no treatment; HG: high glucose (30 mM glucose); PA: palmitic acid sodium (400 μM); Oxymatrine: 10 μM. Data are presented as mean ± SD and represent an average of three experiments. Nrf2, nuclear factor (erythroid-derived 2)-like 2 protein; HO-1, heme oxygenase-1; Keap1, Keleh-like ECH-associated protein. Data are normalized to control. *p < 0.05 vs. control, **p < 0.01 vs. control, #p < 0.05 vs. HG + PA. ##p < 0.01 vs. HG + PA; the experiments were repeated three times.
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