Fig. 4. Oxymatrine affected NF-κB (p65) levels in INS-1 cells at different times.
(A) Representative Western blotting bands of p65 proteins in the nucleus or cytoplasm were showed. (B) Analysis of the amount of p65 proteins in the nucleus when the cells were incubated for 0.5 h. (C) Analysis of the amount of p65 proteins in the cytoplasm when the cells were incubated for 0.5 h. (D) Analysis of the amount of p65 proteins in the nucleus when the cells were incubated for 1 h. (E) Analysis of the amount of p65 proteins in the cytoplasm when the cells were incubated for 1 h. (F) Analysis of the amount of p65 proteins in the nucleus when the cells were incubated for 2 h. (G) Analysis of the amount of p65 proteins in the cytoplasm when the cells were incubated for 2 h. Control: no treatment; HG: high glucose (30 mM glucose); PA: palmitic acid sodium (400 μM); Oxymatrine: 10 μM. Data are presented as mean ± SD and represent an average of three experiments. NF-κB, nuclear factor kappa B. Data are normalized to control. **p < 0.01 vs. control, ##p < 0.01 vs. HG + PA; the experiments were repeated three times.
© Korean J Physiol Pharmacol
