Fig. 2. Oxymatrine decreased the levels of NLRP3, Gsdmd-N, caspase-1, ASC, IL-1β, and IL-18 in INS-1 cells under high glucose and high fat conditions.
(A) Representative Western blotting bands of NLRP3, Gsdmd-N, caspase-1, and IL-1β proteins were showed. (B) Analysis of the amount of NLRP3 proteins. (C) Analysis of the amount of caspase-1 proteins. (D) Analysis of the amount of IL-1β proteins. (E) Analysis of the amount of Gsdmd-N proteins. (F) Representative western blotting bands of ASC proteins were showed; Oxymatrine: 10 μM. (G) Analysis of the amount of ASC proteins; Oxymatrine: 10 μM. (H) Analysis of the secretion of IL-1β and IL-18; Oxymatrine: 10 μM. (I) Analysis of the activity of caspase-1; Oxymatrine: 10 μM. control: no treatment; HG: high glucose (30 mM glucose); PA: palmitic acid sodium (400 μM). Data are presented as mean ± SD and represent an average of three experiments. NLRP3, Nod-like receptor family pyrin domain containing 3; Gsdmd, Gasdermin D; caspase-1, cysteine aspartic acid-specific proteinase-1; ASC, apoptosis-associated speck-like protein containing a CARD; IL, interleukin. Data from Western blotting are normalized to control. **p < 0.01 vs. control, #p < 0.05, ##p < 0.01 vs. HG + PA; the experiments were repeated three times.
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