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Fig. 4. DSF impairs the metabolic activity and differentiation of GM-CSF-treated BMs. BMs (1 × 106 cells/ml) were incubated in 96-well culture plates and treated with DSF at the indicated concentrations (0–0.5 µM) in the absence or presence of 10 ng/ml GM-CSF for 3 days. The metabolic activity of BMs was measured using the CCK-8 assay (A). Data are expressed as the mean ± SD. To investigate the differentiation to DCs, BMs treated with GM-CSF and DSF were stained with DC-specific markers, CD11c. The percentage of CD11c-positive cells after gating a region based on forward scatter (FSC) and side scatter (SSC) was presented (B). DSF, disulfiram; GM-CSF, granulocyte-macrophage colony-stimulating factor; BMs, bone marrow cells; CCK-8, Cell Counting Kit-8; DC, dendritic cell; O.D., optical density. **, ##, ### indicate p < 0.01 and 0.001 compared to the control BMs in the absence or presence of GM-CSF, respectively.
Korean J Physiol Pharmacol 2022;26:157-164
© Korean J Physiol Pharmacol