Fig. 2. DSF diminishes the MMP and causes the death of BMs.
BMs were cultured in 6-well culture plates (1 × 106 cells/ml) and treated with DSF at the indicated concentrations (0–5 µM). To measure MMP, treated BMs were stained with rhodamine 123 solution. The number in the histograms indicates mean fluorescence intensity (MFI) (A). The control group was set to 100% and the MFI of each histogram was calculated (B). The cells were treated for 2 days and stained with annexin V-FITC/PI. The cells in quadrants indicate necrosis (upper left), late apoptosis (upper right), early apoptosis (lower right), and viable (lower left) cells. The number in quadrants indicates the percentage of cells (C) and the percentage of PI-positive cells was presented (D). DSF, disulfiram; MMP, mitochondrial membrane potential; BMs, bone marrow cells; V-FITC, V-fluorescein isothiocyanate; PI, propidium iodide. *, **Indicate p < 0.05, 0.01 compared to the control (DSF concentration 0 μM), respectively.
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