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Fig. 4. Effect of IMP on intracellular accumulation of Rho 123. (A) Effect of time on the fluorescence intensity of Rho 123 in K562/DOX cells. (B) Effect of the coincubation time of Rho 123 and drugs on the fluorescence intensity of Rho 123 in K562/DOX cells. (C) HPLC method specificity. (C-a) K562/DOX cells solution. (C-b) K562/DOX cells solution containing Rho 123 reference solution. (C-c) K562/DOX cells solution after incubation with Rho 123 for 75 min. (D) Effect of IMP on intracellular accumulation of Rho 123 in K562 cells and K562/DOX cells. K562/DOX cells treated with 10.2 and 20.4 ┬Ámol/L VER, which used as the positive control. Data are shown as means ┬▒ SD, n = 3. IMP, imperatorin; DOX, doxorubicin; Taxol, Taxinol; HPLC, high-performance liquid chromatography; VER, verapamil. **p < 0.01 vs. K562 cells. ##p < 0.01 vs. K562/DOX cells untreated group.
Korean J Physiol Pharmacol 2022;26:145-155 https://doi.org/10.4196/kjpp.2022.26.3.145
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