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Fig. 4. The role of the specific NAMPT inhibitor, FK866, on differentiation in MDPC-23. qRT-PCR was performed to detect the expression levels of BSP (A), ALP (B), Col-1 (C), DMP-1 (D), DSPP (E), and NAMPT (F). GAPDH served as the experimental control. All data are based on three independent experiments. (G) Treatment with FK866 at 1 nM according to the defined conditions. Western blotting was used to assess the expression level of odontoblast biomarkers. Quantitative data for protein expressions (G, left panel) were analyzed by using ImageJ software after β-actin normalization (G, right panel). Each data point represents the mean ± SEM of three experiments. MDPC-23, mouse dental papilla cell-23; BSP, bone sialoprotein; ALP, alkaline phosphatase; Col-1, collagen type-1; DMP-1, dentin matrix protein-1; DSPP, dentin sialophosphoprotein; NAMPT, nicotinamide phosphoribosyltransferase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. *p < 0.05, **p < 0.01, ***p < 0.001.
Korean J Physiol Pharmacol 2022;26:37-45
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