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Fig. 2. The effects of Visfatin on mineralization in MDPC-23 cells. (A) MDPC-23 cell viability after Visfatin treatment as determined by MTT assays. (B) Alizarin Red S stain (upper panel) showing mineralized nodule formation in MDPC-23 cells, cultured in differentiation media and treated with Visfatin for up to 10 days. The mineralization was quantified using colorimetric spectrophotometry (lower panel). (C) Treatment with Visfatin at 50 ng/ml according to the defined conditions. The expression level of odontoblast biomarkers were assayed by Western blotting according to standard methods. Quantitative data for protein expressions (C, upper panel) were analyzed by using ImageJ software after β-actin normalization (C, lower panel). Each data point represents the mean ± SEM of three experiments. MDPC-23, mouse dental papilla cell-23; DSPP, dentin sialophosphoprotein; DMP-1, dentin matrix protein-1; NAMPT, nicotinamide phosphoribosyltransferase; BSP, bone sialoprotein. *p < 0.05, **p < 0.01, ***p < 0.001.
Korean J Physiol Pharmacol 2022;26:37-45 https://doi.org/10.4196/kjpp.2022.26.1.37
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