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Fig. 1. The expression of NAMPT during odontoblastic differentiation of MDPC-23 cells. (A) Mineralized nodule formation in MDPC-23 cells. MDPC-23 cells were cultured with odontoblastic induction medium for 10 days, and mineralization was evaluated by alizarin Red S staining (upper panel). The mineralization was quantified by colorimetric spectrophotometry (lower panel). (B) mRNAs were prepared on day 0, 4, 7, and 10 after induction. qPCR was performed to detect the expression levels of ALP, Col-1, DMP-1, DSPP, BSP, and NAMPT. Quantitative data for mRNA expressions (B, upper panel) were analyzed by using ImageJ software after GAPDH normalization (B, lower panel). Each data point represents the mean ± SEM of three experiments. NAMPT, nicotinamide phosphoribosyltransferase; MDPC-23, mouse dental papilla cell-23; BSP, bone sialoprotein; DMP-1, dentin matrix protein-1; DSPP, dentin sialophosphoprotein; ALP, alkaline phosphatase; Col-1, collagen type-1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. *p < 0.05, **p < 0.01, ***p < 0.001.
Korean J Physiol Pharmacol 2022;26:37-45 https://doi.org/10.4196/kjpp.2022.26.1.37
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