Fig. 4. S-nitrosylation involves carbon monoxide (CO)–induced activation of delayed rectifier K⁺ currents (I_K) in human cardiac fibroblasts (HCFs). (A) Original recordings for the effect of carbon monoxide-releasing molecule-3 (CORM3, 10 μM) on I_K after pretreatment with N-ethylmaleimide (NEM, a thiol-alkylating reagent, 0.5 mM) at +30 mV stimulation. (B) Summarized current–voltage (I–V) curves for the effect of CORM3 (10 μM) on I_K after pretreatment with NEM (0.5 mM). (C) Bar graphs showing the current density changes for the CORM3 effects on I_K after pretreatment with NEM (at +30 mV, n = 7 each). (D–F) The reversing effect of DL-dithiothreitol (DTT, a reducing agent, 5 mM) for CORM3-induced activation on I_K (*p < 0.05 compared to control, ^#p < 0.05 compared to CORM3, n = 7 each).

Korean J Physiol Pharmacol 2022;26:25-36 https://doi.org/10.4196/kjpp.2022.26.1.25

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