Fig. 2. Nitric oxide synthase (NOS) blockers inhibits carbon monoxide (CO)–induced activation of delayed rectifier K⁺ currents (I_K) in human cardiac fibroblasts (HCFs). Currents are obtained in which cells were repeatedly depolarized from −80 mV to +50 mV (400 ms duration). carbon monoxide-releasing molecule-3 (CORM3) was applied either to untreated cells or to cells pretreated for 20 min with NOS blockers. (A) Representative original recordings of I_K show the CORM3 (10 μM) effect after pretreatment of L-N^G-monomethyl arginine citrate (L-NMMA, 100 μM) for 20 min. (B) Summarized current–voltage (I–V) relationship curves for I_K and (C) bar graphs for the summary of current density changes of I_K (at +30 mV) also show the effect of CORM3 (10 μM) on I_K after pretreatment of L-NMMA (100 μM). (D) Representative original recordings of I_K. (E) Summarized I–V relationship curves of I_K, and (F) bar graphs for the summary of current density changes of I_K (at +30 mV) also show the CORM3 (10 μM) effect on I_K after L-N^G-nitroarginine methyl ester (L-NAME, 100 μM) pretreatment (n = 7 each). Values are mean ± SEM (n = 7 each).

Korean J Physiol Pharmacol 2022;26:25-36 https://doi.org/10.4196/kjpp.2022.26.1.25

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