Fig. 6. Uptake and intracellular localization of the PAMAMG3 derivatives.
(A) GBL-14 cells and (B) human dermal fibroblasts (HDFs) were treated with PAMAMG3, PAMAMG3-KRRR, and PAMAMG3-HKRRR labeled with Alexa Fluor 546 and pJDK or pJDK-apoptin at the ratio of 4:1 for 24 h. After 24 h post-exposure, the images were obtained by confocal microscopy. Red fluorescence indicated DNA-labeled with Alexa Fluor 546 dye and blue fluorescence indicated nuclei stained with DAPI. (C, D) Each cell was exposed to Alexa Fluor 488-labeled PAMAMG3, PAMAMG3-KRRR, and PAMAMG3-HKRRR with pJDK or pJDK-apoptin at the ratio of 4:1 for 24 h. After 24 h post-exposed, the cells were harvested. The live cells were stained with LysoTracker Red for lysosomes and counterstained with DAPI for nuclei.
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