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Fig. 2. Characterization of endothelial functional activation by EPC and EC surface markers. The expression of EPC (CD34, c-kit, CXCR4) and EC (Tie2, CD31, CD144) surface markers were analyzed by flow cytometry, and no significant differences were observed. FACS gating was applied using unstained cells as a negative control. The fraction of positively stained cells was determined by comparison with unstained cells. The percentage of positively stained cells is indicated by the positive peaks (red lines show cells stained with each antibody, and black lines show the negative control cells). EPC, endothelial progenitor cell; EC, endothelial cell; FACS, fluorescence-activated cell sorting.
Korean J Physiol Pharmacol 2021;25:459-466
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