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Fig. 5. Pretreatment with sitagliptin prevented autophagy in HUVECs exposed to rapamycin. (A–E) The protein levels of LC3, Beclin-1, LAMP1, and LAMP2 in Sitagliptin-pretreated HUVECs were determined using Western blot after exposed to rapamycin. (F, G) The Cathepsin B/D activity results in Sitagliptin-pretreated HUVECs were determined after exposed to rapamycin. HUVECs, Human umbilical vein endothelial cells; LC3, microtubule-associated protein 1 light chain 3; LAMP1/2, lysosomal-associated membrane protein 1/2. *p < 0.05 vs. RAPA-0.01 group. ***p < 0.001 vs. RAPA-0.01 group, ###p < 0.001 vs. RAPA-0.1 group, &&&p < 0.001 vs. RAPA-1 group, ^^^p < 0.001 vs. RAPA-10 group, $$$p < 0.001 vs. RAPA-100 group.
Korean J Physiol Pharmacol 2021;25:425-437
© Korean J Physiol Pharmacol