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Fig. 5. FC inhibited the activation of NF-κB pathway. (A) The phosphorylation levels of IKK, IκBα and p65 were assessed by Western blot analysis. (B) The fluorescence microscopy imaging was taken. Blue and green indicate nuclei and p65, respectively; scale bar, 50 μm. RAW264.7 macrophages were pretreated with FC for 2 h and then subjected to an stimulation with 1 μg/ml LPS for 18 h. n = 3; data were presented as means ± SD. FC, formosanin C; NF-κB, nuclear factor-kappa B; IKK, IκB kinase; LPS, lipopolysaccharide. *Indicates p < 0.05 vs. the control group; #indicates p < 0.05 vs. the LPS alone group.
Korean J Physiol Pharmacol 2021;25:395-401
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