Fig. 4. Effects of FC on LPS-elicited releases of pro-inflammatory cytokines.
(A–C) The mRNA expression of IL-1β (A), IL-6 (B) and TNF-α (C) was evaluated by qPCR. (D–F) The production of IL-1β (D), IL-6 (E) and TNF-α. (F) was assessed by ELISA assays. RAW264.7 macrophages were pretreated with FC for 2 h and then subjected to an stimulation with 1 μg/ml LPS for 22 h. n = 3; data were presented as means ± SD. FC, formosanin C; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β. *Indicates p < 0.05 vs. the control group; #indicates p < 0.05 vs. the LPS alone group.
© Korean J Physiol Pharmacol
