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Fig. 3. Effects of FC on LPS-elicited PGE production and COX-2 expression. (A) PGE production was examined by ELISA assay. (B) The mRNA levels of COX-2 was assessed by qPCR. (C) The protein levels of COX-2 was determined using Western blot analysis. RAW264.7 macrophages were pretreated with FC for 2 h and then subjected to an stimulation with 1 μg/ml LPS for 22 h. n = 3; data were presented as means ± SD. FC, formosanin C; LPS, lipopolysaccharide; PGE, prostaglandin E2; COX-2, cyclooxygenase-2. *Indicates p < 0.05 vs. the control group; #indicates p < 0.05 vs. the LPS alone group.
Korean J Physiol Pharmacol 2021;25:395-401
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