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Fig. 2. Effects of FC on LPS-elicited NO production and iNOS expression. (A) NO production was examined by Griess assay. (B) The mRNA expression of iNOS was evaluated by qPCR. (C) The protein expression of iNOS was assessed by Western blotting. RAW264.7 macrophages were treated with FC for 2 h and then subjected to an stimulation with 1 μg/ml LPS for 22 h. n = 3; data were presented as means ± SD. FC, formosanin C; LPS, lipopolysaccharide; NO, nitric oxide; iNOS, inducible nitric oxide synthase. *Indicates p < 0.05 vs. the control group; #indicates p < 0.05 vs. the LPS alone group.
Korean J Physiol Pharmacol 2021;25:395-401
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