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Fig. 4. Effect of N-acetyl-p-aminophenol (APAP) treatment on cyclin D1 and cleaved caspase-3 (c Caspase-3) protein expression in the presence or absence of TF-SC and TF-AS. Elevated level of cyclin D1 (arrows) (A) and cleaved caspase-3 (arrows) (C) protein expressions are mostly in the pericentral area in APAP treated animals compared to vehicle treated animals after 24 h. Normal, low, expression of cyclin D1 and cleaved caspase-3 in vehicle treated mice sections. Strong expression of both proteins is detected in hepatocytes around central vein in APAP and APAP + TF-SC-treated treated mice. Low expression of cyclin D1 and cleaved caspase-3 is seen in APAP + TF-AS injected mice. CV, central vein (magnification 200×, scale bar 100 µm). Fluorescence intensity of cyclin D1 (B) and cleaved caspase-3 (D) using ImageJ software (D) of six fields per section for at least six mice per group. aSignificantly different from vehicle treated mice, bSignificantly different from APAP-treated mice, cSignificantly different from APAP + TF-SC treated mice using one-way ANOVA followed by Tukey-Kramer test as multiple comparison at p < 0.05.
Korean J Physiol Pharmacol 2021;25:385-393 https://doi.org/10.4196/kjpp.2021.25.5.385
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