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Fig. 3. Protein expression of RXR-α and RAR-α in mice livers tissues treated with N-acetyl-p-aminophenol (APAP) with or without tissue factor (TF) deoxyoligonucleotides (ODNs). Control group showed basal level of RXR-α expression (arrows), APAP and APAP + TF-SC treated mice displaying an intense speckled RXR-α expression (arrows) around CV in liver. Mice injected with APAP + TF-AS showed normal RXR-α expression around CV (A). Quantitative fluorescence intensity of RXR-α using ImageJ software of six fields per section for at least six mice per group (B). Constitutive expression of RAR-α around central vein in control sections (arrows), APAP treated group and APAP + TF-SC treated mice showing an intense diffuse speckled expression of RAR-α (arrows) around CV. Normal RAR-α expression in tissues of animals injected with APAP + TF-AS (C). Fluorescence intensity of RAR-α using ImageJ software (D). aSignificantly different from vehicle treated mice, bSignificantly different from APAP-treated mice, cSignificantly different from APAP + TF-SC treated mice using one-way ANOVA followed by Tukey-Kramer test as multiple comparison at p < 0.05. CV, central vein (magnification 400×, scale bar 50 µm).
Korean J Physiol Pharmacol 2021;25:385-393
© Korean J Physiol Pharmacol