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Fig. 1. Serum alanine transaminase (ALT) and hepatic tissue factor (TF) expression in N-acetyl-p-aminophenol (APAP)-treated mice in the presence or absence of TF deoxyoligonucleotides (ODNs). (A) Increased serum ALT in APAP treated group (800 mg/kg, single i.p. dose) significantly compared to control vehicle-treated mice (0.2 ml per mice, i.p.). APAP + TF-SC group (3.5 h prior to APAP injection in a dose of 6 mg/kg in 100 µl saline, i.v.) has no significant difference compared to APAP-treated mice. APAP + TF-AS (similar dose as TF-SC) reduced significantly serum ALT level compared to APAP treated mice. Data of the figure are represented as means ± SD. (B) TF protein expression is increased in hepatocytes (arrows) and hepatic stellate cells in the pericentral zone in APAP injected animals. No difference has been detected in APAP + TF-SC compared to APAP treated animals. Low level of expression is seen in APAP + TF-AS sections compared to APAP-treated group. CV, central vein (magnification 200X, scale bar 100 µm and 100X, scale bar 250 µm). (C) TF fluorescence intensity is blotted in bar chart. (D) Quantitative histogram of TF in green fluorescence measuring the intensity of six fields for at least six mice per group. (E) Western blot analysis of liver tissue samples from normal and APAP with or without TF ODNs using TF and β-actin Abs as an internal control (n = 3). Data in the figures are represented as mean ± SD. aSignificantly different from vehicle treated group. bSignificantly different from APAP-treated group using one-way ANOVA followed by Tukey-Kramer test as multiple comparisons (p < 0.05).
Korean J Physiol Pharmacol 2021;25:385-393
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