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Fig. 6. Rescuing effects of β-catenin overexpression on anti-inflammatory effects of Wnt-C59 in RAW264.7 murine macrophage cells. Cells grown in 12 well plate were transfected with control vector plasmid (1.5 μg/well) or β-catenin overexpressing plasmid (1.0 or 1.5 μg/well). After one day, cells were treated with 50 μM of Wnt-C59, followed by lipopolysaccharide (LPS) stimulation at 0.1 μg/ml for 4 h. (A) Messenger RNA level of β-catenin were measured by RT-qPCR. (B) Nuclear factor-kappa B (NF-κB) activity for target DNA binding was measured by ELISA. (C, D) Messenger RNA levels of proinflammatory cytokines were measured by RT-qPCR. **p < 0.01, ***p < 0.001 compared with cells transfected with control plasmid. ###p < 0.001 compared with unstimulated cells. Experiments were conducted in triplicate. Data are shown as mean ± standard deviation, and statistical significance was measured by unpaired t-test.
Korean J Physiol Pharmacol 2021;25:307-319
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