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Fig. 4. Luteolin protects against hydrogen peroxide (H2O2)-induced p53 phosphorylation via sirtuin 1 (SIRT1) protein expression. House Ear Institute-Organ of Corti 1 (HEI-OC1) cells were transfected with 80 nM siRNAs of control or SIRT1, then incubated in 2 μM luteolin for 12 h, followed by 30 μM H2O2 treatment for additional 3 days. (A, B) The protein levels were detected by Western blotting and quantified by densitometry based on immunoblot images. (C) Representative images of SA-β-gal staining of HEI-OC1 (×100). (D) The percentage of senescent cells were calculated in (D). Data represents means values of triple experiments. *p < 0.05 vs. control; #p < 0.05 vs. H2O2-treated cells.
Korean J Physiol Pharmacol 2021;25:297-305
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