Fig. 3. Effects of luteolin on sirtuin 1 (SIRT1) expression in House Ear Institute-Organ of Corti 1 cells (HEI-OC1).
(A, C) HEI-OC1 cells were treated with luteolin (0–4 μM, 12 h). (B, D) Cells were treated with luteolin (2 μM, 0–12 h). The proteins level (A, B) and SIRT1 activity (C, D) were performed with Western blotting and SIRT1 fluorometric kit, respectively. The proteins were quantified by densitometry based on immunoblot images. Data represents means values of triple experiments. Significance vs. control: *p < 0.05.
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