Fig. 2. Protective effect of luteolin against hydrogen peroxide (H2O2)-induced cellular senescence is dependent on p53.
(A) Cells were pretreated with 2 μM luteolin for 12 h, then incubated in 30 μM H2O2 for 3 days. (B) Cells were transfected with various concentrations siRNAs of control or p53, then incubated with 30 μM H2O2 for 3 days. The protein levels were detected by Western blotting and quantified by densitometry based on immunoblot images (A, B). (C) Representative images of SA-β-gal staining of HEI-OC1 (×100). (D) The percentage of senescent cells were calculated in (D). Data represents means values of triple experiments. *p < 0.05 vs. control; #p < 0.05 vs. H2O2-treated cells.
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