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Fig. 1. Luteolin reduces hydrogen peroxide (H2O2)-induced cellular senescence in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells. (A–C) Cells were pretreated with luteolin for 12 h, then incubated in 30 μM H2O2 for 3 days. Cell viability was determined by MTT assay (A). The percentage of SA-β-gal positive cells out of total cells was counted and the average data was obtained from three independent experiments (B). The senescent phenotype of HEI-OC1 cells was detected by the SA-β-gal assay (×100) (C). (D) Cell growth was evaluated by MTT assay at various time points indicated in the figure after addition of H2O2. Data represents means values of triple experiments. *p < 0.05 vs. control, #p < 0.05 vs. H2O2. Piperine was used as a positive control.
Korean J Physiol Pharmacol 2021;25:297-305
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