Fig. 4. Inhibitory effects of fargesin, eudesmin, and magnolin on CD3/CD28 co-stimulated human CD⁴⁺ T lymphocyte activation. (A) Fargesin toxicity to Jurkat T cell line after administration of 1, 3, 10, 30, and 100 μM based on CCK-8 assay. (B) Eudesmin cytotoxicity to Jurkat T cell at concentrations in (A). (C) Cytotoxic effects of magnolin to Jurkat T cell at concentrations in (A). (D) Representative figure illustrating proliferation of human naïve CD4⁺ T cells treated with three constituents, and percentage T cell growth inhibition determined by carboxyfluorescein succinimidyl ester (CFSE). Human naïve CD4⁺ T cells stimulated with antibodies CD3 and CD28 (negative control); CD3/CD28-stimulated cells treated with 10 μM BTP2 (positive control). (E) Statistical analysis of percentage CD4⁺ T cell proliferation with constituent and BTP2 treatments in cells stimulated or not stimulated by CD3/CD28. ****p < 0.0001 vs. the control.

Korean J Physiol Pharmacol 2021;25:251-258 https://doi.org/10.4196/kjpp.2021.25.3.251

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