Fig. 5. Effect of S-nitrosylation on carbon monoxide (CO)-induced IBK activation.
(A) Current–voltage (I–V) curves from –60 to +50 mV and (B) bar graphs at +50 mV showing the summarized current density changes by CO-releasing molecule (CORM)-2 (10 μM, n = 6) or CORM-3 (10 μM, n = 6) on IBK after pre-treatment with N-ethylmaleimide (NEM, a thiol-alkylating reagent, 0.5 mM). (C) I–V curves and (D) bar graphs showing the summarized current density changes for the effect of CORM-2 (n = 6) or CORM-3 (n = 6) on IBK after pre-treatment with DL-dithiothreitol (DTT, a reducing agent; 5 mM). *p < 0.05, **p < 0.01 vs. the control, #p < 0.05 vs. CORMs.
© Korean J Physiol Pharmacol
