Fig. 3. Effect of cGMP signaling pathways on carbon monoxide (CO)-induced IBK activation in human cardiac fibroblasts (HCFs).
(A) Representative currents and current–voltage (I–V) curves show the summarized current density changes for the effect of 300 μM 8-Br-cGMP on IBK, n = 8, **p < 0.01 vs. the control. (B) Bar graphs show the summary of the current density changes regarding the effect of 8-Br-cGMP (300 μM) at +50 mV. Values are mean ± SEM. (C) I–V curves and (D) bar graphs also showing the summarized current density changes for the effect of pre-treatment with KT5823 (1 μM, a PKG blocker) for 20 min for IBK activation induced by CO-releasing molecule (CORM)-2 (10 μM, n = 12) or CORM-3 (10 μM, n = 8). (E) I–V curves and (F) bar graphs also showing the summarized current density changes for the effect of 10 μM CORM-2 (n = 12) or 10 μM CORM-3 (n = 8) on IBK after pre-treatment with 1 μM ODQ, a specific soluble guanylate cyclase (sGC) blocker.
© Korean J Physiol Pharmacol
