Fig. 4. Migration and ruffle formation of bone marrow-derived macrophages were dependent on the IDH2 expression.
(A) Representative pictures of bright field microscopy of cultured bone marrow-derived macrophages of IDH2+/+ and IDH2−/− mice. (B) Representative pictures of bright field microscopy of H&E-stained migrated bone marrow-derived macrophages of IDH2+/+ and IDH2−/− mice. Macrophages stained pink. Small circles are pores of the membrane filters. (C) The graph summarizes the number of migrated macrophages. Data are presented as mean ± SE (n = 3). **p < 0.01 vs. IDH2+/+. (D) Representative images of immunofluorescence staining for IDH2 (green), Rac1 (red), DAPI for nuclear staining (blue), and merge.
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