Fig. 5. Effects of gamma-aminobutyric acid (GABA)-salt on the expression and function of GABAB receptor in endothelial cell (ECs).
To identify changes in GABAB receptors and in peNOS and eNOS, media, salt or GABA-salt treated ECs and aorta of hypertensive mice were subjected to qRT-PCR and immunofluorescence. (A) The qRT-PCR graph showing the mRNA expressions of GABAB receptor in media, salt or GABA-salt treated SVEC 4–10. (B, C) Immunofluorescent images showing eNOS and peNOS (green, nuclei; blue) expressions in (B) SVEC 4–10 and in (C) the aorta of hypertensive mice (brown, arrows). Scale bar = 50 µm. (D) Immunofluorescent images showing the expression of the adherent proteins such as E-selectin, VCAM-1, ICAM-1 and vWF (green, nuclei; blue) in the aorta of hypertensive mice. Scale bar = 50 µm. (E, F) Apoptotic cells are detected by TUNEL analysis. The fluorescent images and graph showing apoptotic cell in the aorta of hypertensive mice. Scale bar = 50 µm. ICAM-1, Intercellular Adhesion Molecule-1; VCAM-1, vascular cell adhesion molecule-1; vWF, von Willebrand Factor. *p < 0.05, and ***p < 0.001 vs. culture media or normal diet (ND); and $p < 0.05, and $$$p < 0.001 vs. salt or high cholesterol diet (HCD)/salt.
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