Fig. 4. Apigenin-induced oxidative stress in MSTO-211H and H-2452 cells. Cells were pretreated with or without 5 mM NAC for 2 h prior to treatment with 30 μM apigenin for 48 h. (A) Cellular ROS levels were measured by staining cells with 10 μM DCF-DA. (B) Cell cycle distribution was determined by flow cytometry following staining with propidium iodide (20 μg/ml). (C) Apoptotic cell fraction was analyzed using annexin V-PE binding assay. (D) ΔΨm was measured by staining cells with 30 nM rhodamine123. APG, apigenin; NAC, N-acetylcysteine; Arrows, sub-G0/G1 peak. #p < 0.05 vs. group treated with APG alone.
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