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Fig. 2. Apigenin-induced apoptosis and necroptosis in MSTO-211H and H-2452 cells. (A) Cells were treated with increasing concentrations (0, 10, 30, and 50 μM) of apigenin for 48 h. Levels of apoptosis- and necroptosis-related proteins were assessed by Western blotting. (B) Cells were pretreated with 25 μM necrostatin-1 and 10 μM Q-VD-Oph-1 2 h prior to treatment with apigenin (30 and 50 μM) for 48 h and 72 h. Cell viability was measured by MTT assay. APG, apigenin. *p < 0.05 vs. respective control group. #p < 0 .05 vs. group treated with APG alone.
Korean J Physiol Pharmacol 2020;24:493-502
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