Fig. 1. Apigenin-induced cytotoxicity. Two MM cell lines, MSTO-211H and H-2452, and normal mesothelial cell line MeT-5A were treated with increasing concentrations (0, 10, 30, and 50 μM) of apigenin or cisplatin for 48 h and 72 h. (A) Cell viability was measured by MTT assay. (B) Cell cycle distribution was determined by flow cytometry following staining with propidium iodide (20 μg/ml). (C) Apoptotic cell fraction was analyzed using annexin V-PE binding assay. (D) Levels of DNA damage response proteins were assessed by Western blotting. MM, malignant mesothelioma; APG, apigenin; 7-AAD, 7-amino-actinomycin D; Arrows, sub-G0/G1 peak. *p < 0.05 vs. respective control group.
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