Fig. 6. Effects of high fructose intake on the expression of renin-angiotensin system (RAS) genes in the adrenal glands. (A, B) RAS-related gene expression was quantified by RT-qPCR. However, high fructose intake did not affect the expression of angiotensin II type 1a receptor (AT1aR) or angiotensin II type 1b receptor (AT1bR) in the adrenal glands. Representative images show adrenals from rats drinking either water (control, n = 6) or water containing 20% fructose (fructose, n = 6). (C) Adrenal gland sections were stained with H&E. AT1R and angiotensin II (Ang II) proteins were detected by immunohistochemistry. The graphs show the mean ± standard error of three independent experiments (*p = 0.05, **p = 0.01, control vs. fructose).
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