Fig. 2. S109 treatment decreased the invasive ability of U87 and U251 cells. (A, C) U87 and U251 cells were treated with vehicle or S109 (0.25 μM, 0.5 μM, and 1.0 μM) for 30 h, and then cell invasion was assessed by transwell invasion assay. The invasive cells were stained with a 0.1% crystal violet solution for 30 min. (B, D) Quantitation of numbers of cells that invaded through the filter. The number of invading cells was counted and normalized to the control group. The data are expressed as the means ± standard error of the mean from three independent experiments, ***p < 0.001.
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